Professor Elena Klenova

Yasinska, IM., Sakhnevych, SS., Pavlova, L., Teo Hansen Selnø, A., Teuscher Abeleira, AM., Benlaouer, O., Gonçalves Silva, I., Mosimann, M., Varani, L., Bardelli, M., Hussain, R., Siligardi, G., Cholewa, D., Berger, SM., Gibbs, BF., Ushkaryov, YA., Fasler-Kan, E., Klenova, E. and Sumbayev, VV., (2019). The Tim-3-Galectin-9 Pathway and Its Regulatory Mechanisms in Human Breast Cancer. Frontiers in Immunology. 10 (JULY), 1594-

Pavlaki, I., Docquier, F., Chernukhin, I., Kita, G., Gretton, S., Clarkson, CT., Teif, VB. and Klenova, E., (2018). Poly(ADP-ribosyl)ation associated changes in CTCF-chromatin binding and gene expression in breast cells. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms. 1861 (8), 781-730

Venkatraman, B. and Klenova, E., (2015). Role of CTCF poly(ADP-Ribosyl)ation in the regulation of apoptosis in breast cancer cells. Indian Journal of Medical and Paediatric Oncology. 36 (01), 49-54

Cheema, Z., Hari‐Gupta, Y., Kita, G., Farrar, D., Seddon, I., Corr, J. and Klenova, E., (2014). Expression of the cancer‐testis antigen BORIS correlates with prostate cancer. The Prostate. 74 (2), 164-176

Méndez-Catalá, CF., Gretton, S., Vostrov, A., Pugacheva, E., Farrar, D., Ito, Y., Docquier, F., Kita, G-X., Murrell, A., Lobanenkov, V. and Klenova, E., (2013). A Novel Mechanism for CTCF in the Epigenetic Regulation of Bax in Breast Cancer Cells. Neoplasia. 15 (8), 898-IN14

Guastafierro, T., Catizone, A., Calabrese, R., Zampieri, M., Martella, O., Bacalini, MG., Reale, A., Di Girolamo, M., Miccheli, M., Farrar, D., Klenova, E., Ciccarone, F. and Caiafa, P., (2013). ADP-ribose polymer depletion leads to nuclear Ctcf re-localization and chromatin rearrangement. Biochemical Journal. 449 (3), 623-630

Chernukhin, I., Kang, SY., Brown, S., Gretton, S., Mendez-Catala, CF., Cowieson, D. and Klenova, E., (2011). BioVyon Protein A, an alternative solid-phase affinity matrix for chromatin immunoprecipitation. Analytical Biochemistry. 412 (2), 183-188

Farrar, D., Chernukhin, I. and Klenova, E., (2011). Generation of Poly(ADP-ribosyl)ation Deficient Mutants of the Transcription Factor, CTCF. Methods in molecular biology (Clifton, N.J.). 780, 293-312

Ohlsson, R., Lobanenkov, V. and Klenova, E., (2010). Does CTCF mediate between nuclear organization and gene expression?. BioEssays. 32 (1), 37-50

Farrar, D., Rai, S., Chernukhin, I., Jagodic, M., Ito, Y., Yammine, S., Ohlsson, R., Murrell, A. and Klenova, E., (2010). Mutational Analysis of the Poly(ADP-Ribosyl)ation Sites of the Transcription Factor CTCF Provides an Insight into the Mechanism of Its Regulation by Poly(ADP-Ribosyl)ation. Molecular and Cellular Biology. 30 (5), 1199-1216

Ali, FR., Vasiliou, SA., Haddley, K., Paredes, UM., Roberts, JC., Miyajima, F., Klenova, E., Bubb, VJ. and Quinn, JP., (2010). Combinatorial interaction between two human serotonin transporter gene variable number tandem repeats and their regulation by CTCF. Journal of Neurochemistry. 112 (1), 296-306

Docquier, F., Kita, G-X., Farrar, D., Jat, P., O'Hare, M., Chernukhin, I., Gretton, S., Mandal, A., Alldridge, L. and Klenova, E., (2009). Decreased Poly(ADP-Ribosyl)ation of CTCF, a Transcription Factor, Is Associated with Breast Cancer Phenotype and Cell Proliferation. Clinical Cancer Research. 15 (18), 5762-5771

Ito, Y., Koessler, T., Ibrahim, AEK., Rai, S., Vowler, SL., Abu-Amero, S., Silva, A-L., Maia, A-T., Huddleston, JE., Uribe-Lewis, S., Woodfine, K., Jagodic, M., Nativio, R., Dunning, A., Moore, G., Klenova, E., Bingham, S., Pharoah, PDP., Brenton, JD., Beck, S., Sandhu, MS. and Murrell, A., (2008). Somatically acquired hypomethylation of IGF2 in breast and colorectal cancer. Human Molecular Genetics. 17 (17), 2633-2643

D'Arcy, V., Pore, N., Docquier, F., Abdullaev, ZK., Chernukhin, I., Kita, G-X., Rai, S., Smart, M., Farrar, D., Pack, S., Lobanenkov, V. and Klenova, E., (2008). BORIS, a paralogue of the transcription factor, CTCF, is aberrantly expressed in breast tumours. British Journal of Cancer. 98 (3), 571-579

D'Arcy, V., Pore, N., Docquier, F., Abdullaev, ZK., Chernukhin, I., Kita, G-X., Rai, S., Smart, M., Farrar, D., Pack, S., Lobanenkov, V. and Klenova, E., (2008). Erratum: BORIS, a paralogue of the transcription factor, CTCF, is aberrantly expressed in breast tumours. British Journal of Cancer. 98 (3), 676-676

Hancock, AL., Brown, KW., Moorwood, K., Moon, H., Holmgren, C., Mardikar, SH., Dallosso, AR., Klenova, E., Loukinov, D., Ohlsson, R., Lobanenkov, VV. and Malik, K., (2007). A CTCF-binding silencer regulates the imprinted genes AWT1 and WT1-AS and exhibits sequential epigenetic defects during Wilms' tumourigenesis. Human Molecular Genetics. 16 (3), 343-354

Roberts, J., Scott, AC., Howard, MR., Breen, G., Bubb, VJ., Klenova, E. and Quinn, JP., (2007). Differential Regulation of the Serotonin Transporter Gene by Lithium Is Mediated by Transcription Factors, CCCTC Binding Protein and Y-Box Binding Protein 1, through the Polymorphic Intron 2 Variable Number Tandem Repeat. The Journal of Neuroscience. 27 (11), 2793-2801

Chernukhin, I., Shamsuddin, S., Kang, SY., Bergström, R., Kwon, Y-W., Yu, W., Whitehead, J., Mukhopadhyay, R., Docquier, F., Farrar, D., Morrison, I., Vigneron, M., Wu, S-Y., Chiang, C-M., Loukinov, D., Lobanenkov, V., Ohlsson, R. and Klenova, E., (2007). CTCF Interacts with and Recruits the Largest Subunit of RNA Polymerase II to CTCF Target Sites Genome-Wide. Molecular and Cellular Biology. 27 (5), 1631-1648

D'Arcy, V., Abdullaev, ZK., Pore, N., Docquier, F., Torrano, V., Chernukhin, I., Smart, M., Farrar, D., Metodiev, M., Fernandez, N., Richard, C., Delgado, MD., Lobanenkov, V. and Klenova, E., (2006). The Potential of BORIS Detected in the Leukocytes of Breast Cancer Patients as an Early Marker of Tumorigenesis. Clinical Cancer Research. 12 (20), 5978-5986

Torrano, V., Navascués, J., Docquier, F., Zhang, R., Burke, LJ., Chernukhin, I., Farrar, D., León, J., Berciano, MT., Renkawitz, R., Klenova, E., Lafarga, M. and Delgado, MD., (2006). Targeting of CTCF to the nucleolus inhibits nucleolar transcription through a poly(ADP-ribosyl)ation-dependent mechanism. Journal of Cell Science. 119 (9), 1746-1759

Torrano, V., Chernukhin, I., Docquier, F., D'Arcy, V., León, J., Klenova, E. and Delgado, MD., (2005). CTCF Regulates Growth and Erythroid Differentiation of Human Myeloid Leukemia Cells. Journal of Biological Chemistry. 280 (30), 28152-28161

Docquier, F., Farrar, D., D'Arcy, V., Chernukhin, I., Robinson, AF., Loukinov, D., Vatolin, S., Pack, S., Mackay, A., Harris, RA., Dorricott, H., O'Hare, MJ., Lobanenkov, V. and Klenova, E., (2005). Heightened Expression of CTCF in Breast Cancer Cells Is Associated with Resistance to Apoptosis. Cancer Research. 65 (12), 5112-5122

Klenova, E. and Ohlsson, R., (2005). Poly(ADP-ribosyl)ation and Epigenetics: Is CTCF PARt of the Plot?. Cell Cycle. 4 (1), 96-101

El-Kady, A. and Klenova, E., (2005). Regulation of the transcription factor, CTCF, by phosphorylation with protein kinase CK2. FEBS Letters. 579 (6), 1424-1434

Yu, W., Ginjala, V., Pant, V., Chernukhin, I., Whitehead, J., Docquier, F., Farrar, D., Tavoosidana, G., Mukhopadhyay, R., Kanduri, C., Oshimura, M., Feinberg, AP., Lobanenkov, V., Klenova, E. and Ohlsson, R., (2004). Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation. Nature Genetics. 36 (10), 1105-1110

Pant, V., Kurukuti, S., Pugacheva, E., Shamsuddin, S., Mariano, P., Renkawitz, R., Klenova, E., Lobanenkov, V. and Ohlsson, R., (2004). Mutation of a Single CTCF Target Site within the H19 Imprinting Control Region Leads to Loss of Igf2 Imprinting and Complex Patterns of De Novo Methylation upon Maternal Inheritance. Molecular and Cellular Biology. 24 (8), 3497-3504

Klenova, E., Scott, AC., Roberts, J., Shamsuddin, S., Lovejoy, EA., Bergmann, S., Bubb, VJ., Royer, H-D. and Quinn, JP., (2004). YB-1 and CTCF Differentially Regulate the 5-HTT Polymorphic Intron 2 Enhancer Which Predisposes to a Variety of Neurological Disorders. The Journal of Neuroscience. 24 (26), 5966-5973

Mukhopadhyay, R., Yu, W., Whitehead, J., Xu, J., Lezcano, M., Pack, S., Kanduri, C., Kanduri, M., Ginjala, V., Vostrov, A., Quitschke, W., Chernukhin, I., Klenova, E., Lobanenkov, V. and Ohlsson, R., (2004). The Binding Sites for the Chromatin Insulator Protein CTCF Map to DNA Methylation-Free Domains Genome-Wide. Genome Research. 14 (8), 1594-1602

Klenova, E., (2002). Immunoprecipitation techniques for the analysis of transcription factor complexes. Methods. 26 (3), 254-259

Filippova, GN., Ulmer, JE., Moore, JM., Ward, MD., Hu, YJ., Neiman, PE., Collins, SJ., Qi, CF., Loukinov, DI., Pugacheva, EM., Morse, HC., Lobanenkov, VV., Klenova, EM., Grundy, PE., Feinberg, AP., Cleton-Jansen, AM., Moerland, EW., Cornelisse, CJ., Suzuki, H., Komiya, A., Lindblom, A. and Dorion-Bonnet, F., (2002). Tumor-associated zinc finger mutations in the CTCF transcription factor selectively alter its DNA-binding specificity. Cancer Research. 62 (1), 48-52

Klenova, EM., Morse, HC., Ohlsson, R. and Lobanenkov, VV., (2002). The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer. Seminars in Cancer Biology. 12 (5), 399-414

Loukinov, DI., Pugacheva, E., Vatolin, S., Pack, SD., Moon, H., Chernukhin, I., Mannan, P., Larsson, E., Kanduri, C., Vostrov, AA., Cui, H., Niemitz, EL., Rasko, JEJ., Docquier, FM., Kistler, M., Breen, JJ., Zhuang, Z., Quitschke, WW., Renkawitz, R., Klenova, EM., Feinberg, AP., Ohlsson, R., Morse, HC. and Lobanenkov, VV., (2002). BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma. Proceedings of the National Academy of Sciences. 99 (10), 6806-6811

Klenova, EM., Chernukhin, IV., El-Kady, A., Lee, RE., Pugacheva, EM., Loukinov, DI., Goodwin, GH., Delgado, D., Filippova, GN., León, J., Morse, HC., Neiman, PE. and Lobanenkov, VV., (2001). Functional Phosphorylation Sites in the C-Terminal Region of the Multivalent Multifunctional Transcriptional Factor CTCF. Molecular and Cellular Biology. 21 (6), 2221-2234

Rasko, JEJ., Klenova, EM., Leon, J., Filippova, GN., Loukinov, DI., Vatolin, S., Robinson, AF., Jia Hu, Y., Ulmer, J., Ward, MD., Pugacheva, EM., Neiman, PE., Morse, HC., Collins, SJ. and Lobanenkov, VV., (2001). Cell growth inhibition by the multifunctional multivalent zinc-finger factor CTCF. Cancer Research. 61 (16), 6002-6007

Chernukhin, IV., Shamsuddin, S., Robinson, AF., Carne, AF., Paul, A., El-Kady, AI., Lobanenkov, VV. and Klenova, EM., (2000). Physical and Functional Interaction between Two Pluripotent Proteins, the Y-box DNA/RNA-binding Factor, YB-1, and the Multivalent Zinc Finger Factor, CTCF. Journal of Biological Chemistry. 275 (38), 29915-29921

Chernukhin, IV. and Klenova, EM., (2000). A Method of Immobilization on the Solid Support of Complex and Simple Enzymes Retaining Their Activity. Analytical Biochemistry. 280 (1), 178-181

Delgado, MD., Chernukhin, IV., Bigas, A., Klenova, EM. and León, J., (1999). Differential expression and phosphorylation of CTCF, a c‐myc transcriptional regulator, during differentiation of human myeloid cells. FEBS Letters. 444 (1), 5-10

Filippova, GN., Lindblom, A., Meincke, LJ., Klenova, EM., Neiman, PE., Collins, SJ., Doggett, NA. and Lobanenkov, VV., (1998). A widely expressed transcription factor with multiple DNA sequence specificity,CTCF, is localized at chromosome segment 16q22.1 within one of the smallest regions of overlap for common deletions in breast and prostate cancers. Genes, Chromosomes and Cancer. 22 (1), 26-36

Klenova, EM., Fagerlie, S., Filippova, GN., Kretzner, L., Goodwin, GH., Loring, G., Neiman, PE. and Lobanenkov, VV., (1998). Characterization of the Chicken CTCF Genomic Locus, and Initial Study of the Cell Cycle-regulated Promoter of the Gene. Journal of Biological Chemistry. 273 (41), 26571-26579

Klenova, E., (1997). Molecular weight abnormalities of the CTCF transcription factor: CTCF migrates aberrantly in SDS-PAGE and the size of the expressed protein is affected by the UTRs and sequences within the coding region of the CTCF gene. Nucleic Acids Research. 25 (3), 466-474

Filippova, GN., Fagerlie, S., Klenova, EM., Myers, C., Dehner, Y., Goodwin, G., Neiman, PE., Collins, SJ. and Lobanenkov, VV., (1996). An Exceptionally Conserved Transcriptional Repressor, CTCF, Employs Different Combinations of Zinc Fingers To Bind Diverged Promoter Sequences of Avian and Mammalian c-myc Oncogenes. Molecular and Cellular Biology. 16 (6), 2802-2813

Klenova, EM., Nicolas, RH., Paterson, HF., Carne, AF., Heath, CM., Goodwin, GH., Neiman, PE. and Lobanenkov, VV., (1993). CTCF, a conserved nuclear factor required for optimal transcriptional activity of the chicken c-myc gene, is an 11-Zn-finger protein differentially expressed in multiple forms.. Molecular and Cellular Biology. 13 (12), 7612-7624

Klenova, EM., Botezato, I., Laudet, V., Goodwin, GH., Wallace, JC. and Lobanenkov, VV., (1992). Isolation of a cDNA clone encoding the RNASE-superfamily-related gene highly expressed in chicken bone marrow cells. Biochemical and Biophysical Research Communications. 185 (1), 231-239

Adler, VV., Kiseleva, NP., Kistanova, EN., Klenova, EM., Lobanenkov, VV., Polotskaya, AV. and Tevosyan, SG., (1991). Differences in expression and functional organization of rat tyrosine aminotransferase gene in two Morris hepatoma cell lines 7777 and 8994. Molekulyarnaya Biologiya. 25 (2), 431-441

Kintsurashvili, EG., Adler, VV., Klenova, EM., Lobanenkov, VV. and Razin, SV., (1990). Multiple DNA recognition sites by sequence-specific DNA-binding proteins in the region of replication initiation site located 5-end upstream to the chicken alpha-globin gene domain. Doklady Akademii nauk SSSR. 310 (1), 227-228

Kintsurashvili, EG., Adler, VV., Klenova, EM., Lobanenkov, VV. and Razin, SV., (1990). Multiple recognition sites for DNA by sequence-specific DNA-binding proteins in the neighborhood of the site of the beginning of replication, situated in the 5-terminal region of the domain of chicken α-globin genes. Doklady Biochemistry. 310 (1-6), 9-11

Kabishev, AA., Klenova, EM., Gribanovskiǐ, VA. and Gorodetskiǐ, SI., (1990). The identification of the binding sites of nuclear factor I in the region of the bovine beta-casein gene. Doklady Akademii nauk SSSR. 315 (4), 997-1000

Lobanenkov, VV., Nicolas, RH., Adler, VV., Paterson, H., Klenova, EM., Polotskaja, AV. and Goodwin, GH., (1990). A novel sequence-specific DNA binding protein which interacts with three regularly spaced direct repeats of the CCCTC-motif in the 5′-flanking sequence of the chicken c-myc gene. Oncogene. 5 (12), 1743-1753

Klenova, EM., Adler, VV. and Lobanenkov, VV., (1989). In vitro formation of multimers of specific DNA-protein complexes with nuclear factor I (NF I). Doklady Akademii nauk SSSR. 306 (4), 995-998

Klenova, EM., Adler, VV., Gribanovsky, VA., Gorodetsky, SI., Bogdanova, NN. and Lobanenkov, VV., (1989). Specific interaction of a nuclear protein factor from the CTF/NF-I family with two distinct promoter regions of the rat tyrosine aminotransferase gene. Molekulyarnaya Biologiya. 23 (5), 1400-1415

Klenova, EM., Polotskaya, AV., Adler, VV., Gorodetsky, SI., Gribanovsky, VA. and Lobanenkov, VV., (1989). A rat-liver nuclear protein factor A5 that requires metal to interact specifically with the distal promoter element of the tyrosine aminotransferase gene in vitro. Molekulyarnaya Biologiya. 23 (5), 1416-1427

Klenova, EM., Adler, VV. and Lobanenkov, VV., (1989). In vitro formation of multimers of specific DNA-protein complexes with nuclear factor I (NF I). Doklady Biochemistry. 306 (1-6), 157-160

Lobanenkov, VV., Klenova, EM., Gorodetskii, SI. and Adler, VV., (1988). DNA-binding nuclear protein factors that interact specifically with the elements of the promoter of the rat tyrosine aminotransferase gene. Doklady Biochemistry. 298 (1-6), 11-16

Lobanenkov, VV., Klenova, EM., Gorodetskiǐ, SI. and Adler, VV., (1988). DNA-binding nuclear protein factors specifically binding with the promoter elements of the tyrosine aminotransferase gene in the rat. Doklady Akademii nauk SSSR. 298 (3), 746-750

Klenova, EM., Desiatnikov, OO., Bulachev, VN. and Gorodetskiǐ, SI., (1988). Change in the level of casein mRNA in the mammary gland of rats and goats during lactation. Biokhimiya. 53 (11), 1925-1931