Professor Jonathan Worrall
![Professor Jonathan Worrall](http://www1.essex.ac.uk/people-images/WORRA41301.jpg)
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Email
jworrall@essex.ac.uk -
Telephone
+44 (0) 1206 872095
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Location
3SW.5.23, Colchester Campus
Profile
Biography
2007-present: University of Essex, UK 2005-2007: Post-doctoral research associate, Department of Biochemistry, University of Cambridge, UK 1999-2005: Post-doctoral research associate, Leiden Institute of Chemistry, Leiden University, The Netherlands 1995-1999: PhD in Bioinorganic Chemistry, Department of Chemistry, Newcastle University, UK 1992-1995: BSc (Hons) Medicinal Chemistry, Newcastle University, UK Local Ambassador for the Biochemical Society Secretary of the Inorganic Biochemistry Discussion Group, (Royal Society of Chemistry)
Research and professional activities
Research interests
Microbial Biotechnology
Copper homeostasis in Streptomyces
Lipid induced apoptosis
Structural Biochemistry
Current research
My research focus is on the role of metal ions and metalloproteins in biology.
Copper and StreptomycesWe are interested in the role of copper on the morphological development of the antibiotic producing actinobacterium Streptomyces lividans. Using microbial genetics, structural biology, spectroscopy and RNA-seq analysis we have identified and characterised copper sensing and trafficking pathways leading to the metallation of cuproenzymes that are critical for initiating development in this industrially important microbe.
Mitochondrial cytochrome c and apoptosisThe haem containing redox protein cytochrome c is a key mitochondrial protein that plays an important role in the life and death decisions of a cell. In the mitochondrial apoptosis pathway cytochrome c is released from the mitochondria into the cytosol leading to the formation of the apoptosome assembly, a cellular platform to initiate caspase activity triggering cell death. The selective oxidation by cytochorme c of a unique inner-membrane mitochondrial phospholipid, diphosphatidylglycerol, better known as cardiolipin has been demonstrated to trigger events that lead to cytochrome c release from the mitochondria. Using NMR and EPR spectroscopies we are investigating the interaction and complex formed bewteen cytochorme c and cardiolipin.
Teaching and supervision
Current teaching responsibilities
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Metals in Biotechnology (BS228)
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Structural and Molecular Enzymology (BS314)
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Industrial Biotechnology (BS937)
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Research Project in Biomedical Science (BS831)
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Research Project in Life Sciences (BS832)
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Employability Skills for the Biosciences (BS211)
Previous supervision
![Peter Charles Smyth](http://www1.essex.ac.uk/people-images/SMYTH52608.jpg?mh=60&)
Degree subject: Biochemistry
Degree type: Doctor of Philosophy
Awarded date: 26/6/2024
![Hannah Ruth Adams](http://www1.essex.ac.uk/people-images/ADAMS73400.jpg?mh=60&)
Degree subject: Biological Sciences
Degree type: Doctor of Philosophy
Awarded date: 16/2/2024
![Mia Louise Alice Hanlon](http://www1.essex.ac.uk/people-images/HANLO61502.jpg?mh=60&)
Degree subject: Biochemistry
Degree type: Master of Science (by Dissertation)
Awarded date: 29/6/2023
![Marina Rozman](http://www1.essex.ac.uk/people-images/LUCIC44507.jpg?mh=60&)
Degree subject: Biochemistry
Degree type: Doctor of Philosophy
Awarded date: 7/2/2022
![Megan Laura Straw](http://www1.essex.ac.uk/people-images/STRAW67303.jpg?mh=60&)
Degree subject: Biological Sciences
Degree type: Doctor of Philosophy
Awarded date: 5/7/2019
![Oliver Mario Deacon](http://www1.essex.ac.uk/people-images/DEACO07104.jpg?mh=60&)
Degree subject: Biochemistry
Degree type: Doctor of Philosophy
Awarded date: 25/6/2018
![Amanda Kay Chaplin](http://www1.essex.ac.uk/people-images/CHAPL85009.jpg?mh=60&)
Degree subject: Biochemistry
Degree type: Doctor of Philosophy
Awarded date: 23/3/2016
![Tatiana De Vasconcelos Porto](http://www1.essex.ac.uk/people-images/PORTO66101.jpg?mh=60&)
Degree subject: Biochemistry
Degree type: Doctor of Philosophy
Awarded date: 21/3/2016
![Katie Laura Irene May Blundell](http://www1.essex.ac.uk/people-images/BLUND45907.jpg?mh=60&)
Degree subject: Biochemistry
Degree type: Doctor of Philosophy
Awarded date: 8/5/2014
![Badri Sreenivasachar Rajagopal](http://www1.essex.ac.uk/people-images/RAJAG10402.jpg?mh=60&)
Degree subject: Biochemistry
Degree type: Doctor of Philosophy
Awarded date: 12/2/2013
Publications
Journal articles (77)
Thompson, AJ., Sanchez-Weatherby, J., Williams, LJ., Mikolajek, H., Sandy, J., Worrall, JAR. and Hough, MA., (2024). Efficient in situ screening of and data collection from microcrystals in crystallization plates. Acta Crystallographica Section D Structural Biology. 80 (4), 279-288
Lučić, M., Allport, T., Clarke, TA., Williams, LJ., Wilson, MT., Chaplin, AK. and Worrall, JAR., (2024). The oligomeric states of dye‐decolorizing peroxidases from Streptomyces lividans and their implications for mechanism of substrate oxidation. Protein Science. 33 (7), e5073-
Bolton, R., Machelett, MM., Worrall, JAR., Hough, MA. and et al., (2024). A redox switch allows binding of Fe(II) and Fe(III) ions in the cyanobacterial iron-binding protein FutA from Prochlorococcus. Proceedings of the National Academy of Sciences of the United States of America. 121 (12), e2308478121-
Hough, MA., Prischi, F. and Worrall, JAR., (2023). Perspective: Structure determination of protein-ligand complexes at room temperature using X-ray diffraction approaches. Frontiers in Molecular Biosciences. 10, 1113762-
Lučić, M., Wilson, MT., Pullin, J., Hough, MA., Svistunenko, DA. and Worrall, JAR., (2023). New insights into controlling radical migration pathways in heme enzymes gained from the study of a dye-decolorising peroxidase. Chemical Science. 14 (44), 12518-12534
Moreno-Chicano, T., Carey, LM., Axford, D., Beale, JH., Doak, RB., Duyvesteyn, HME., Ebrahim, A., Henning, RW., Monteiro, DCF., Myles, DA., Owada, S., Sherrell, DA., Straw, ML., Šrajer, V., Sugimoto, H., Tono, K., Tosha, T., Tews, I., Trebbin, M., Strange, RW., Weiss, KL., Worrall, JAR., Meilleur, F., Owen, RL., Ghiladi, RA. and Hough, MA., (2022). Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature. IUCrJ. 9 (5), 610-624
Worrall, JAR. and Hough, MA., (2022). Serial femtosecond crystallography approaches to understanding catalysis in iron enzymes.. Current Opinion in Structural Biology. 77, 102486-102486
Lučić, M., Wilson, MT., Tosha, T., Sugimoto, H., Shilova, A., Axford, D., Owen, RL., Hough, MA. and Worrall, JAR., (2022). Serial Femtosecond Crystallography Reveals the Role of Water in the One- or Two-Electron Redox Chemistry of Compound i in the Catalytic Cycle of the B-Type Dye-Decolorizing Peroxidase DtpB. ACS Catalysis. 12 (21), 13349-13359
Munir, A., Wilson, MT., Hardwick, SW., Chirgadze, DY., Worrall, JAR., Blundell, TL. and Chaplin, AK., (2021). Using cryo-EM to understand antimycobacterial resistance in the catalase-peroxidase (KatG) from Mycobacterium tuberculosis. Structure. 29 (8), 899-912.e4
Pullin, J., Wilson, MT., Clémancey, M., Blondin, G., Bradley, JM., Moore, GR., Le Brun, NE., Lučić, M., Worrall, JAR. and Svistunenko, DA., (2021). Iron oxidation in Escherichia coli bacterioferritin ferroxidase centre, a site designed to react rapidly with H2O2 but slowly with O2. Angewandte Chemie International Edition. 60 (15), 8361-8369
Lučić, M., Wilson, MT., Svistunenko, DA., Owen, RL., Hough, MA. and Worrall, JAR., (2021). Aspartate or arginine? Validated redox state X-ray structures elucidate mechanistic subtleties of FeIV = O formation in bacterial dye-decolorizing peroxidases. Journal of Biological Inorganic Chemistry. 26 (7), 743-761
Horrell, S., Axford, D., Devenish, NE., Ebrahim, A., Hough, MA., Sherrell, DA., Storm, SLS., Tews, I., Worrall, JAR. and Owen, RL., (2021). Fixed Target Serial Data Collection at Diamond Light Source.. Journal of Visualized Experiments. 2021 (168)
Deacon, OM., White, RW., Moore, GR., Wilson, MT. and Worrall, JAR., (2020). Comparison of the structural dynamic and mitochondrial electron-transfer properties of the proapoptotic human cytochrome c variants, G41S, Y48H and A51V. Journal of Inorganic Biochemistry. 203, 110924-110924
Lucic, M., Chaplin, AK., Moreno-Chicano, T., Dworkowski, F., Wilson, M., Svistunenko, D., Hough, M. and Worrall, JAR., (2020). A subtle structural change in the distal haem pocket has a remarkable effect on tuning hydrogen peroxide reactivity in dye decolourising peroxidases from Streptomyces lividans. Dalton Transactions. 49 (5), 1620-1636
Lucic, M., Svistunenko, D., Wilson, M., Chaplin, A., Davy, B., Ebrahim, A., Axford, D., Tosha, T., Sugimoto, H., Owada, S., Dworkowski, F., Tews, I., Owen, R., Hough, M. and Worrall, JAR., (2020). Serial femtosecond zero dose crystallography captures a water‐free distal heme site in a dye‐decolourising peroxidase to reveal a catalytic role for an arginine in FeIV=O formation. Angewandte Chemie International Edition. 59 (48), 21656-21662
Lučić, M., Svistunenko, DA., Wilson, MT., Chaplin, AK., Davy, B., Ebrahim, A., Axford, D., Tosha, T., Sugimoto, H., Owada, S., Dworkowski, FSN., Tews, I., Owen, RL., Hough, MA. and Worrall, JAR., (2020). Serial Femtosecond Zero Dose Crystallography Captures a Water‐Free Distal Heme Site in a Dye‐Decolorising Peroxidase to Reveal a Catalytic Role for an Arginine in FeIV=O Formation. Angewandte Chemie. 132 (48), 21840-21846
Chaplin, AK., Chicano, TM., Hampshire, BV., Wilson, MT., Hough, MA., Svistunenko, DA. and Worrall, JAR., (2019). An Aromatic Dyad Motif in Dye Decolourising Peroxidases Has Implications for Free Radical Formation and Catalysis. Chemistry – A European Journal. 25 (24), 6141-6153
Straw, ML., Hough, MA., Wilson, MT. and Worrall, JAR., (2019). A histidine residue and a tetranuclear cuprous‐thiolate cluster dominate the copper loading landscape of a copper storage protein from Streptomyces lividans. Chemistry – A European Journal. 25 (45), 10678-10688
Ebrahim, A., Moreno-Chicano, T., Appleby, MV., Chaplin, AK., Beale, JH., Sherrell, DA., Duyvesteyn, HME., Owada, S., Tono, K., Sugimoto, H., Strange, RW., Worrall, JAR., Axford, D., Owen, RL. and Hough, MA., (2019). Dose-resolved serial synchrotron and XFEL structures of radiation-sensitive metalloproteins. IUCrJ. 6 (4), 543-551
Moreno-Chicano, T., Ebrahim, A., Axford, D., Appleby, MV., Beale, JH., Chaplin, AK., Duyvesteyn, HME., Ghiladi, RA., Owada, S., Sherrell, DA., Strange, RW., Sugimoto, H., Tono, K., Worrall, JAR., Owen, RL. and Hough, MA., (2019). High-throughput structures of protein–ligand complexes at room temperature using serial femtosecond crystallography. IUCrJ. 6 (6), 1074-1085
Beale, JH., Bolton, R., Marshall, SA., Beale, EV., Carr, SB., Ebrahim, A., Moreno-Chicano, T., Hough, MA., Worrall, JAR., Tews, I. and Owen, RL., (2019). Successful sample preparation for serial crystallography experiments. Journal of Applied Crystallography. 52 (6), 1385-1396
Straw, ML., Chaplin, AK., Hough, MA., Paps, J., Bavro, VN., Wilson, MT., Vijgenboom, E. and Worrall, JAR., (2018). A cytosolic copper storage protein provides a second level of copper tolerance in Streptomyces lividans.. Metallomics: integrated biometal science. 2018 (10), 180-193
Deacon, OM., Svistunenko, DA., Moore, GR., Wilson, MT. and Worrall, JAR., (2018). Naturally Occurring Disease-Related Mutations in the 40–57 Ω-Loop of Human Cytochrome c Control Triggering of the Alkaline Isomerization. Biochemistry. 57 (29), 4276-4288
Chaplin, AK., Svistunenko, DA., Hough, MA., Wilson, MT., Vijgenboom, E. and Worrall, JAR., (2017). Active site maturation and activity of the copper-radical oxidase GlxA is governed by a tryptophan residue. The Biochemical Journal. 474 (5), 809-825
Karsisiotis, AI., Deacon, OM., Macdonald, C., Blumenschein, TMA., Moore, GR. and Worrall, JAR., (2017). Near-complete backbone resonance assignments of acid-denatured human cytochrome c in dimethylsulfoxide: a prelude to studying interactions with phospholipids. Biomolecular NMR Assignments. 11 (2), 165-168
Chaplin, AK., Bernini, C., Sinicropi, A., Basosi, R., Worrall, JAR. and Svistunenko, DA., (2017). Tyrosine or Tryptophan? Modifying a Metalloradical Catalytic Site by Removal of the Cys–Tyr Cross‐Link in the Galactose 6‐Oxidase Homologue GlxA. Angewandte Chemie International Edition. 129 (23), 6602-6606
Kekilli, D., Moreno-Chicano, T., Chaplin, AK., Horrell, S., Dworkowski, FSN., Worrall, JAR., Strange, RW. and Hough, MA., (2017). Photoreduction and validation of haem?ligand intermediate states in protein crystals by in situ single-crystal spectroscopy and diffraction. IUCrJ. 4 (3), 263-270
Chaplin, AK., Wilson, MT. and Worrall, JAR., (2017). Kinetic characterisation of a dye decolourising peroxidase from Streptomyces lividans: New insight into the mechanism of anthraquinone dye decolourisation. Dalton Transactions. 46 (29), 9420-9429
Deacon, OM., Karsisiotis, AI., Moreno-Chicano, T., Hough, MA., Macdonald, C., Blumenschein, TMA., Wilson, MT., Moore, GR. and Worrall, JAR., (2017). Heightened Dynamics of the Oxidized Y48H Variant of Human Cytochrome c Increases Its Peroxidatic Activity.. Biochemistry. 56 (46), 6111-6124
Chaplin, AK., Bernini, C., Sinicropi, A., Basosi, R., Worrall, JAR. and Svistunenko, DA., (2017). Tyrosine or Tryptophan? Modifying a Metalloradical Catalytic Site by Removal of the Cys–Tyr Cross‐Link in the Galactose 6‐Oxidase Homologue GlxA. Angewandte Chemie. 129 (23), 6602-6606
Chaplin, AK., Wilson, MT., Hough, MA., Svistunenko, DA., Hemsworth, GR., Walton, PH., Vijgenboom, E. and Worrall, JAR., (2016). Heterogeneity in the Histidine-brace Copper Coordination Sphere in Auxiliary Activity Family 10 (AA10) Lytic Polysaccharide Monooxygenases. Journal of Biological Chemistry. 291 (24), 12838-12850
Petrus, MLC., Vijgenboom, E., Chaplin, AK., Worrall, JAR., van Wezel, GP. and Claessen, D., (2016). The DyP-type peroxidase DtpA is a Tat-substrate required for GlxA maturation and morphogenesis in Streptomyces. Open Biology. 6 (1), 150149-150149
Karsisiotis, AI., Deacon, OM., Wilson, MT., Macdonald, C., Blumenschein, TMA., Moore, GR. and Worrall, JAR., (2016). Increased dynamics in the 40–57 Ω-loop of the G41S variant of human cytochrome c promote its pro-apoptotic conformation. Scientific Reports. 6 (1), 30447-
Chaplin, AK., Tan, BG., Vijgenboom, E. and Worrall, JAR., (2015). Copper trafficking in the CsoR regulon of Streptomyces lividans. Metallomics. 7 (1), 145-155
Chaplin, AK., Petrus, MLC., Mangiameli, G., Hough, MA., Svistunenko, DA., Nicholls, P., Claessen, D., Vijgenboom, E. and Worrall, JAR., (2015). GlxA is a new structural member of the radical copper oxidase family and is required for glycan deposition at hyphal tips and morphogenesis of Streptomyces lividans. Biochemical Journal. 469 (3), 433-444
Porto, TV., Hough, MA. and Worrall, JAR., (2015). Structural insights into conformational switching in the copper metalloregulator CsoR fromStreptomyces lividans. Acta Crystallographica Section D Biological Crystallography. 71 (9), 1872-1878
Karsisiotis, AI., Deacon, OM., Rajagopal, BS., Macdonald, C., Blumenschein, TMA., Moore, GR. and Worrall, JAR., (2015). Backbone resonance assignments of ferric human cytochrome c and the pro-apoptotic G41S mutant in the ferric and ferrous states. Biomolecular NMR Assignments. 9 (2), 415-419
Porto, TV., Wilson, MT. and Worrall, JAR., (2015). Copper and nickel bind via two distinct kinetic mechanisms to a CsoR metalloregulator. Dalton Transactions. 44 (46), 20176-20185
Hough, MA., Silkstone, G., Worrall, JAR. and Wilson, MT., (2014). NO Binding to the Proapoptotic Cytochrome c–Cardiolipin Complex. NITRIC OXIDE. 96, 193-209
Blundell, KLIM., Hough, MA., Vijgenboom, E. and Worrall, JAR., (2014). Structural and mechanistic insights into an extracytoplasmic copper trafficking pathway in Streptomyces lividans. Biochemical Journal. 459 (3), 525-538
Tan, BG., Vijgenboom, E. and Worrall, JAR., (2014). Conformational and thermodynamic hallmarks of DNA operator site specificity in the copper sensitive operon repressor from Streptomyces lividans. Nucleic Acids Research. 42 (2), 1326-1340
Rajagopal, BS., Edzuma, AN., Hough, MA., Blundell, KLIM., Kagan, VE., Kapralov, AA., Fraser, LA., Butt, JN., Silkstone, GG., Wilson, MT., Svistunenko, DA. and Worrall, JAR., (2013). The hydrogen-peroxide-induced radical behaviour in human cytochrome c–phospholipid complexes: implications for the enhanced pro-apoptotic activity of the G41S mutant. Biochemical Journal. 456 (3), 441-452
Blundell, KLIM., Wilson, MT., Svistunenko, DA., Vijgenboom, E. and Worrall, JAR., (2013). Morphological development and cytochrome c oxidase activity in Streptomyces lividans are dependent on the action of a copper bound Sco protein. Open Biology. 3 (1), 120163-120163
Blundell, KLIM., Wilson, MT., Vijgenboom, E. and Worrall, JAR., (2013). The role of the Cys-X-X-X-Cys motif on the kinetics of cupric ion loading to the Streptomyces lividans Sco protein. Dalton Transactions. 42 (29), 10608-10608
Dwarakanath, S., Chaplin, AK., Hough, MA., Rigali, S., Vijgenboom, E. and Worrall, JAR., (2012). Response to Copper Stress in Streptomyces lividans Extends beyond Genes under Direct Control of a Copper-sensitive Operon Repressor Protein (CsoR). Journal of Biological Chemistry. 287 (21), 17833-17847
Svistunenko, DA., Worrall, JAR., Chugh, SB., Haigh, SC., Ghiladi, RA. and Nicholls, P., (2012). Ferric haem forms of Mycobacterium tuberculosis catalase-peroxidase probed by EPR spectroscopy: Their stability and interplay with pH. Biochimie. 94 (6), 1274-1280
Mason, JM., Bendall, DS., Howe, CJ. and Worrall, JAR., (2012). The role of a disulfide bridge in the stability and folding kinetics of Arabidopsis thaliana cytochrome c6A. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1824 (2), 311-318
Rajagopal, BS., Silkstone, GG., Nicholls, P., Wilson, MT. and Worrall, JAR., (2012). An investigation into a cardiolipin acyl chain insertion site in cytochrome c. Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1817 (5), 780-791
Bischin, C., Deac, F., Silaghi-Dumitrescu, R., Worrall, JAR., Rajagopal, BS., Damian, G. and Cooper, CE., (2011). Ascorbate peroxidase activity of cytochromec. Free Radical Research. 45 (4), 439-444
Worrall, JAR. and Mason, JM., (2011). Thermodynamic analysis of Jun-Fos coiled coil peptide antagonists. FEBS Journal. 278 (4), 663-672
Rajagopal, BS., Wilson, MT., Bendall, DS., Howe, CJ. and Worrall, JAR., (2011). Structural and kinetic studies of imidazole binding to two members of the cytochrome c 6 family reveal an important role for a conserved heme pocket residue. JBIC Journal of Biological Inorganic Chemistry. 16 (4), 577-588
Volkov, AN., Nicholls, P. and Worrall, JAR., (2011). The complex of cytochrome c and cytochrome c peroxidase: The end of the road?. Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1807 (11), 1482-1503
Worrall, JAR. and Vijgenboom, E., (2010). Copper mining in Streptomyces: enzymes, natural products and development. Natural Product Reports. 27 (5), 742-742
Volkov, AN., Bashir, Q., Worrall, JAR., Ullmann, GM. and Ubbink, M., (2010). Shifting the Equilibrium between the Encounter State and the Specific Form of a Protein Complex by Interfacial Point Mutations. Journal of the American Chemical Society. 132 (33), 11487-11495
Volkov, AN., Bashir, Q., Worrall, JAR. and Ubbink, M., (2009). Binding Hot Spot in the Weak Protein Complex of Physiological Redox Partners Yeast Cytochrome c and Cytochrome c Peroxidase. Journal of Molecular Biology. 385 (3), 1003-1013
Worrall, JAR., Górna, M., Crump, NT., Phillips, LG., Tuck, AC., Price, AJ., Bavro, VN. and Luisi, BF., (2008). Reconstitution and analysis of the multienzyme Escherichia coli RNA degradosome.. Journal of Molecular Biology. 382 (4), 870-883
Worrall, JAR., Howe, FS., McKay, AR., Robinson, CV. and Luisi, BF., (2008). Allosteric Activation of the ATPase Activity of the Escherichia coli RhlB RNA Helicase. Journal of Biological Chemistry. 283 (9), 5567-5576
Worrall, JAR., Luisi, BF., Schlarb-Ridley, BG., Bendall, DS. and Howe, CJ., (2008). Cytochrome c6A: discovery, structure and properties responsible for its low haem redox potential. Biochemical Society Transactions. 36 (6), 1175-1179
Worrall, JAR. and Luisi, BF., (2007). Information available at cut rates: structure and mechanism of ribonucleases. Current Opinion in Structural Biology. 17 (1), 128-137
Worrall, JAR., Górna, M., Pei, XY., Spring, DR., Nicholson, RL. and Luisi, BF., (2007). Design and chance in the self-assembly of macromolecules. Biochemical Society Transactions. 35 (3), 502-507
Worrall, JAR., Schlarb-Ridley, BG., Reda, T., Marcaida, MJ., Moorlen, RJ., Wastl, J., Hirst, J., Bendall, DS., Luisi, BF. and Howe, CJ., (2007). Modulation of Heme Redox Potential in the Cytochrome c6 Family. Journal of the American Chemical Society. 129 (30), 9468-9475
Volkov, AN., Worrall, JAR., Holtzmann, E. and Ubbink, M., (2006). Solution structure and dynamics of the complex between cytochromecand cytochromecperoxidase determined by paramagnetic NMR. Proceedings of the National Academy of Sciences. 103 (50), 18945-18950
Marcaida, MJ., Schlarb-Ridley, BG., Worrall, JAR., Wastl, J., Evans, TJ., Bendall, DS., Luisi, BF. and Howe, CJ., (2006). Structure of Cytochrome c6A, a Novel Dithio-cytochrome of Arabidopsis thaliana, and its Reactivity with Plastocyanin: Implications for Function. Journal of Molecular Biology. 360 (5), 968-977
Worrall, JAR., Machczynski, MC., Keijser, BJF., di Rocco, G., Ceola, S., Ubbink, M., Vijgenboom, E. and Canters, GW., (2006). Spectroscopic Characterization of a High-Potential Lipo-Cupredoxin Found in Streptomyces coelicolor. Journal of the American Chemical Society. 128 (45), 14579-14589
(2006). CORRECTION. Protein Science. 15 (6), 1563-1563
Volkov, AN., Ferrari, D., Worrall, JAR., Bonvin, AMJJ. and Ubbink, M., (2006). The orientations of cytochrome c in the highly dynamic complex with cytochrome b5 visualized by NMR and docking using HADDOCK (vol 14, pg 799, 2005). PROTEIN SCIENCE. 15 (6), 1563-1563
Worrall, JAR., van Roon, AM., Ubbink, M. and Canters, GW., (2005). The effect of replacing the axial methionine ligand with a lysine residue in cytochrome c‐550 from Paracoccus versutus assessed by X‐ray crystallography and unfolding. The FEBS Journal. 272 (10), 2441-2455
Volkov, AN., Ferrari, D., Worrall, JAR., Bonvin, AMJJ. and Ubbink, M., (2005). The orientations of cytochrome c in the highly dynamic complex with cytochrome b5 visualized by NMR and docking using HADDOCK. Protein Science. 14 (3), 799-811
Worrall, JAR., Diederix, REM., Prudêncio, M., Lowe, CE., Ciofi‐Baffoni, S., Ubbink, M. and Canters, GW., (2005). The Effects of Ligand Exchange and Mobility on the Peroxidase Activity of a Bacterial Cytochrome c upon Unfolding. ChemBioChem. 6 (4), 747-758
Diederix, REM., Fittipaldi, M., Worrall, JAR., Huber, M., Ubbink, M. and Canters, GW., (2003). Kinetic Stability of the Peroxidase Activity of Unfolded Cytochrome c: Heme Degradation and Catalyst Inactivation by Hydrogen Peroxide. Inorganic Chemistry. 42 (22), 7249-7257
Worrall, JAR., Reinle, W., Bernhardt, R. and Ubbink, M., (2003). Transient Protein Interactions Studied by NMR Spectroscopy: The Case of Cytochrome c and Adrenodoxin. Biochemistry. 42 (23), 7068-7076
Crowley, PB., Rabe, KS., Worrall, JAR., Canters, GW. and Ubbink, M., (2002). The Ternary Complex of Cytochrome f and Cytochrome c: Identification of a Second Binding Site and Competition for Plastocyanin Binding. ChemBioChem. 3 (6), 526-526
Ubbink, M., Worrall, JAR., Canters, GW., Groenen, EJJ. and Huber, M., (2002). Paramagnetic Resonance of Biological Metal Centers. Annual Review of Biophysics and Biomolecular Structure. 31 (1), 393-422
Worrall, JAR., Liu, Y., Crowley, PB., Nocek, JM., Hoffman, BM. and Ubbink, M., (2002). Myoglobin and Cytochrome b5: A Nuclear Magnetic Resonance Study of a Highly Dynamic Protein Complex. Biochemistry. 41 (39), 11721-11730
Crowley, PB., Rabe, KS., Worrall, JAR., Canters, GW. and Ubbink, M., (2002). The ternary complex of cytochrome f and cytochrome c: Identification of a second binding site and competition for plastocyanin binding. ChemBioChem. 3 (6), 526-533
Worrall, JAR., Kolczak, U., Canters, GW. and Ubbink, M., (2001). Interaction of Yeast Iso-1-cytochrome c with Cytochrome c Peroxidase Investigated by [15N,1H] Heteronuclear NMR Spectroscopy. Biochemistry. 40 (24), 7069-7076
Im, S-C., Worrall, JAR., Liu, G., Aliverti, A., Zanetti, G., Luchinat, C., Bertini, I. and Sykes, AG., (2000). The CrIIL Reduction of [2Fe-2S] Ferredoxins and Site of Attachment of CrIII Using 1H NMR and Site-Directed Mutagenesis. Inorganic Chemistry. 39 (8), 1755-1764
Book chapters (1)
Worrall, JAR., (2021). Copper Storage Protein From Streptomyces Lividans. In: Encyclopedia of Inorganic and Bioinorganic Chemistry (EIBC). Editors: Scott, RA., . Wiley. 1- 16. 9781119951438
Conferences (3)
Karsisiotis, AI., Deacon, OM., Wilson, MT., Macdonald, C., Blumenschein, TMA., Moore, GR. and Worrall, JAR., (2017). The G41S Variant of Human Cytochrome C Enhances Apoptosis via Increased Dynamics
Ubbink, M., Crowley, PB. and Worrall, JAR., (2001). Dynamics of electron transfer protein complexes probed by NMR
Worrall, JAR., Volkov, A., Canters, GW. and Ubbink, M., (2001). Heme protein-protein interactions studied by NMR
Grants and funding
2023
A holistic approach to reaction initiation at XFEL and synchrotron facilities
Biotechnology and Biological Sciences Research Council
TRICSS - a multi-user high-throughput platform to quantify biological interactions in solution
Biotechnology and Biological Sciences Research Council
TRICSS - a multi-user high-throughput platform to quantify biological interactions in solution
Biotechnology and Biological Sciences Research Council
2021
Proton management in the stabilisation of tyrosyl radicals in peroxidases
Royal Society of Chemistry
Breaking the Cage: Transformative Time-resolved Crystallography using Fixed Targets at Synchrotrons and XFELs
Biotechnology and Biological Sciences Research Council
Breaking the Cage: Transformative Time-resolved Crystallography using Fixed Targets at Synchrotrons and XFELs
Biotechnology and Biological Sciences Research Council
Breaking the Cage: Transformative Time-resolved Crystallography using Fixed Targets at Synchrotrons and XFELs
Biotechnology and Biological Sciences Research Council
2020
Replacement EPR spectrometer to the multiuser Biomedical EPR Facility at the University of Essex
Biotechnology and Biological Sciences Research Council
2019
Protein Detection Kit
East Suffolk and North Essex NHS Foundation Trust
2018
High hit-rate, high-throughput serial protein crystallography at SACLA using fixed target silicon nitride chips
Biotechnology and Biological Sciences Research Council
2017
The role of carbohydrate binding modules appended to lytic polysaccharide monooxygenases on substrate binding
Biochemical Society
The role of carbohydrate binding modules appended to lytic polysaccharide monooxygenases on substrate binding
Biochemical Society
Creating new starch active copper LPMOs through the generation of loop libraries
Biotechnology and Biological Sciences Research Council
2013
Characterisation of a protein bound free radical induced by hydrogen peroxide
Wellcome Trust
Fat(al) attraction of cytochrome c: A new approach to study protein-lipid interaction
Leverhulme Trust
2012
Elucidating the maturation pathway of a cuproenzyme proposed to be critical for motphological development in Steptomyces lividans
Society of Biology
2011
Summer Vacation Studentship - Amanda Chaplin
Biochemical Society
2008
Protein Interactions Between A Dead-Box
The Royal Society